Applied Biosystems™ QuantStudio™ 7 Pro RealTime PCR System, 384well
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other QuantStudio instruments. Figure 2. Comparison of ΔΔC t data between QuantStudio 7 Pro, QuantStudio 7 Flex, and 7900HT systems demonstrates equivalent relative expression level of GFAP, ALB, and KRT1 in cDNA of universal human reference RNA, total liver RNA (LIV), and total brain (BRN) RNA. A day in the life of a qPCR pro. At your desk. The QuantStudio 7 Pro system accommodates the interchange of 96-well (0.2 mL), 96-well (0.1 mL), 384-well, and TaqMan array card (TAC) blocks. The system is delivered with one block type; other block types may be purchased separately. In addition, an Orbitor Microplate Mover is available for this system to support high-throughput workflows.
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Standard Operation Procedure for QuantStudio Author: Genomics Facility, Cornell Institute of Biotechnology Subject: Step by step protocol to use the qPCR instrument QuantStudio 7 Pro Keywords: Protocol, Basic usage, Instrument setup Created Date: 12/3/2020 5:10:43 PM. The QuantStudio 7 Pro system can detect 1.5 fold changes in expression. A dilution series of the KAZ plasmid was amplified. The vertical axis is the change in fluorescence normalized to the ROX dye. The horizontal axis is increasing cycle number from left to right. This is verified by plotting as a standard curve on the right.