for cDNA synthesis use sythesis Kit for e.g. the BioRad iScript cDNA synthesis Kit Cat.# 1708891 use 200 ng of RNA for reaction the reaction protocol reverse transcription was carried out at 42° C for 30 min The reverse transcriptaseinactivation was performed at 85° C for 5 min used directly or stored at 20° C. iScript ™ Select cDNA Synthesis Kit 25 x 20 µl reactions 170-8896 100 x 20 µl reactions 170-8897 For research purposes only Store at -20°C The iScript Select cDNA synthesis kit is a sensitive, flexible, and easy-to-use kit for the generation of first-strand cDNA. This robust reverse transcription kit allows a selection of first-strand priming strategies , oligo (dT) primers only, random.
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iScript™ cDNA Synthesis Kit Catalog # Description 1708890 iScript cDNA Synthesis Kit, 25 x 20 µl reactions 1708891 iScript cDNA Synthesis Kit, 100 x 20 µl reactions For research purposes only. Introduction Component Volume per Reaction, µl iScript cDNA Synthesis Kit provides a sensitive and easy-to- 5x iScript Reaction Mix 4 use solution for two-step reverse transcription quantitative.. Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples. Key Features and Benefits Up to 15µl of RNA in a 20µl cDNA synthesis reaction Combined oligo (dT) and random primers provide unbiased representation of 5 and 3 regions Fast 26 min cDNA synthesis protocol Linear range of 1µg-1 pg total input RNA using.
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